Microarray - An Introduction to Bioinformatics Algorithms...

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Probing DNA May, 11, 2004 1 An Introduction to Bioinformatics Algorithms www.bioalgorithms.info
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DNA Hybridization Single-stranded DNA will naturally bind to complementary strands. Hybridization is used to locate genes, measure the level of gene expression, and determine the degree of similarity between DNA from different sources. Hybridization is also referred to as annealing or re-naturation. May, 11, 2004 2 An Introduction to Bioinformatics Algorithms www.bioalgorithms.info
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Molecular Biology Laboratory Techniques :Hybridization Given a short (8- to 30-nucleotides or oligonucleotides) synthetic fragment DNA, called probe , a target single stranded DNA molecule (produced by denaturing) will hybridize or bind to the probe if there is a substring in the target sequence that is complementary to the probe. For example, a target DNA sequence CCCTGGCACCTA will hybridize to a probe ACCGTGGA since the complementary sequence TGGCACCT is present in the target. In the mix of a DNA, the presence of a particular DNA can be tested by making the probe fluorescent or radioactive. This idea has lead to the development of DNA chips or microarrays that allows rapid DNA sequencing.
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Create a Hybridization Reaction 1. Hybridization is binding two genetic sequences. The binding occurs because of the hydrogen bonds [pink] between base pairs. 2.
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Microarray - An Introduction to Bioinformatics Algorithms...

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