File-013 - BIO 119 File-013 McCague Exercise II Purpose The...

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BIO 119 McCague File-013 Exercise II Purpose: The preparation and study of a differential white count of human blood. Materials: 1. Hemolets 2. Clean, dry slides 3. Alcohol and cotton 4. Wright’s stain 5. Distilled water 6. Prepared frog blood smears Procedure: A. Two slides are thoroughly cleaned , then dried. A finger tip is prepared for puncture by alcohol and then dried with cotton. Sterile hemolets are provided for puncture. The drop of blood obtained is placed at the end of one clean slide. The drop should not be so small as to be scarcely discernible nor represent many drops. After the drop of blood ahs been placed at the end of one clean glass slide, the second clean glass slide is brought up to it and held at an angle of about 30 o and used to drag the drop over the slide and make a smear. If the slide is clean, and the action quick enough, an excellent smear can be obtained. After the smear has dried, cover it with Wright’s stain. Permit the stain to remain for 2 minutes. Add enough distilled water
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This note was uploaded on 06/13/2011 for the course BIO 119 taught by Professor Stevendroho during the Spring '11 term at Moraine Valley Community College.

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File-013 - BIO 119 File-013 McCague Exercise II Purpose The...

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