BIO 119
McCague
File-016
Experiment IV
A.
Effect of temperature in the rate of a biological process.
Temperature is one of the important variables affecting animate and inanimate
processes alike. In the present experiment the effect of temperature on a beating
frog heart may give you some insight into the relation between temperature and
speed of reaction.
Materials:
1. 250 to 400 m. beaker (2)
4. Ice cold Ringer’s Solution
2. Ring stand, etc.
5. Frog’s Ringer’s Solution
3. Dissecting instruments
(room temperature)
Experimental procedure:
Pith the brain and spinal cord of a frog and expose the heart. Be sure to keep the
tissues irrigated with Ringer’s Solution to prevent drying out and deterioration.
Remove the pericardium a thin whitish membrane surrounding the heart, and
identify: a single muscular ventricle, 2 lateral thin walled auricles, and the large
blood artery known as the bulbus arteriosus. Lift the tip of the ventricle so that
you can observe the dorsal side known as the frenum. Cut this with the scissors
and tip the heart forward. You will see a triangular chamber, the sinus venosus,
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- Spring '11
- StevenDroho
- Microbiology, heart rate, Ringer, sinus venosus, Ringer’s solution
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