File-055 - BIO-119 File-055 Biochemical Tests SIM media

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BIO-119 File-055 Biochemical Tests SIM media (Sulfide-Indole-Motility) Motile Non- motile Inoculate carefully with a wire needle Ineubate do not touch glass for 48 – 96 hrs. at bottom of media Differential media Results for detecting H 2 S If H 2 S present note gas, Indole from black ppt. FeS . tryptophan and If indole produced motility red layer forms on top after adding Kovac’s Reagent. Nitrate Broth Add several Inoculate drops of Nitrate with loop Incubate solution A and of bacteria for 48 – 96 hrs. Nitrate solution B. Red color development indicates NO 3 - reduced NO 2 - by bacteria positive result. Different Test for the Reduction of NO 3 - to NO 2 -
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BIO-119 File-055 Further Differential Media D.C.A. EMB Desoxycholate – citrate agar This media can be used in place used to isolate enteric of MacConkey’s Agar. bacteria of the family Indicators are Eosiny and Methylene Enterobacteriaceae – when in Blue. mixed culture,
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This note was uploaded on 06/13/2011 for the course BIO 119 taught by Professor Stevendroho during the Spring '11 term at Moraine Valley Community College.

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File-055 - BIO-119 File-055 Biochemical Tests SIM media

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