finlabpractical - MCAGBIO3.119-disk 227 MORAINE VALLEY...

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MCAGBIO3.119-disk 227 MORAINE VALLEY COMMUNITY COLLEGE BIO 119, Lab Final T. McCague ANSWER ALL QUESTIONS ON ANSWER SHEET . 1. After performing a streak plate for bacterial colony isolation, the plate should be incubated with the lid facing the ceiling. a. true b. false 2. Indole is the breakdown product of: a. acetoin. d. tryptophan. b. catalase. e. glycerol. c. 2, 3-butanediol. 3. MR-VP media contains the carbohydrate: a. lactose. d. sucrose. b. mannitol. e. fructose. c. glucose. 4. Methyl red, the indicator for the MR test turns under acidic or positive result conditions. a. red d. blue b. yellow e. green c. black 5. If bacteria can ferment lactose, it can also ferment glucose. a. true b. false 6. If bacteria can ferment glucose, it can also ferment lactose. a. true b. false 7. Fermentation leaves end products that are energy rich and organic. a. true b. false
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2 BIO 119, Lab Final/MCAGBIO3.119-DISK 227 8. T.S.I. media must be read at: a. 6-10 hours after incubation. b. 24-48 hours after incubation. c. 48-72 hours after incubation. d. anytime after incubation. 9. a. Methyl Red Test. d. Voges-Proskauer Test. b. Nitrate Reduction. e. none of these. c. Indole Test. Matching : (10-14) a. Barritt's reagent b. Kovac's reagent c. nitrite test reagent d. starch hydrolysis reagent e. lactose fermentation indicator 10. Indole test chemicals includes: amyl alcohol, p-dimethyl amino benzaldehyde and HCl. 11. Alpha naphthol and potassium hydroxide are used in combination for this test. 12. Gram's iodine. 13. Phenol red is most often used. 14. This test uses a photo chemical dimethyl-a-naphthylamine, acetic acid, sulfanilic acid and can require the addition of zinc dust. 15. Catalase test media (used to grow bacteria for this test): a. Urea agar d. Nutrient Agar or B.H.I. b. S.I.M. e. Blood agar c. TSA 16. S.I.M. media is used to detect:
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3 BIO 119, Lab Final/MCAGBIO3.119-DISK 227 a. Simmon's citrate utilization. b. motility, tryptophane breakdown and H 2 S gas release. c. sucrose fermentation, mannitol fermentation and H 2 S gas release. d. oxygen requirements, glucose fermentation and H 2 S gas release. e. none of these. 17. When litmus is reduced the media litmus milk turns: a. purple. d. brown. b. pink. e. clear.
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finlabpractical - MCAGBIO3.119-disk 227 MORAINE VALLEY...

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