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MICRO FILE111 - File-111 Lab 34 McCague Oligodynamic Action...

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File-111 McCague Lab 34 Oligodynamic Action 34 melted N.A. talls in water bath 34 sterile petridishes Forceps 10 beakers (50ml) of acid-alcohol 34 sterile water tubes Metal strips copper, nickel, silver, aluminum, zinc 10 N.B. cultures E . coli 10 N.B. cultures Staphlococcus aureus
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File-111 McCague Lab 35 Bacterial Mutagenicity and Carcinogensis: Ames Test 10 N.B. Cultures of TA 98 strain of Salmonella typhimurium 64 plates of glucose-minimal salt agar plates 64 tubes of top agar (2ml/tube) 64 tubes of sterile water Vortex stirrer Forceps 40 sterile Pasteur pipettes + 1ml bulbs 1ml sterile pipettes + bulbs 32 Whatman #1 8mm sterile filter paper in sterile petridish 4-nitro-o- phenylenediamine Test tubes of carcinogens
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File-111 McCague Unknowns – Laboratory 36 random numbered unknowns 001 – 033 Using: Citrobacter freundii Salmonella typhimurium Proteus vulgaris Escherichia coli Pseudomonas aeruginosa Klebsiella pneumoniae 3 Each in Serratia marcescens Nutrient Broth Enterobacter cloacae Staphlococcus aureus Staphlococcus
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