microlabs1 28 - File-090 Lab Exercise 1 McCague Microscope...

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File-090 McCague Lab Exercise 1 Microscope week #1 Small container of table salt NaCl beaker Letter “e” slides – prepared slides Clean slides Cover slip squares Microscopes Video on history of micro. Men of Little Faith or Certain Death Set up 100ml water + navy bean culture under hood cover with foil and Pasteur pipette Note: This is to be used as a reserve culture for slides morphology
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File-090 McCague Lab 5 Survey of Microbes Week #2 class 1 and 2 6 – 10 beakers 250 ml. Label 1, 2, 3, 4, 5, 6 etc. . . Eye droppers Tray of forceps for filamentous algae Clean slides and cover slips Prepared Slides Algae slides –Euglena , Volvox , Mixed Protozoa slides – Paramecium , Amoeba , Mixed Cyanobacteria slides – Anabena , Oscillatoria
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File-090 McCague Lab 7 Aseptic Technique Week #2 class 2 10 Nutrient broth cultures of pigmented Serratia marcescens 32 Nutrient agar slants 32 Nutrient agar plates 32 plates Sabouraud’s agar pH=5.6 or Starch agar is ok
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File-090 McCague Week #3 day1 Lab 8 Bacteria 32 tubes of nutrient broth 32 plates of tryptic soy agar 32 plates of EMB agar or Blood agar if available 60 sterile swabs
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File-090 McCague Week #3 day 2 Lab 9 2 Flasks – 10% sucrose solution with baking yeast Saccharomyces cerevisiae Prepared slides of: yeast budding Saccharomyces cerevisiae Penicillum Aspergillus Rhizopus Clean slides Crystal violet dye Items below needed to make mold cultures or slides 32 sterile swabs 32 empty Petri dishes
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microlabs1 28 - File-090 Lab Exercise 1 McCague Microscope...

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