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protein_engineering - Protein engineering Tailor-made...

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Protein engineering Tailor-made biocatalysts The efficient application of biocatalysts requires the availability of suitable enzymes with high activity and stability under process conditions, desired substrate selectivity and high enantioselectivity Rational (re)design versus directed evolution
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Protein engineering Genetic manipulation techniques Large-scale supply of enzymes at reasonable price Identification of new biocatalysts (screening) doesnot always yield suitable enzymes for a given synthetic problem Computer-aided site-directed mutagenesis Directed (molecular) evolution
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Protein engineering Site-directed mutagenesis Requires structural information and knowledge about relationship between sequence, structure, function and mechanism Very information-intensive Rapid progress in NMR / X-ray methods Genome sequence information Molecular modeling, bioinformatics Prediction of selectivity, activity, stability etc.
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Protein engineering Rational redesign strategy
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