Kyle's Abstract - Copy - Copy - Copy - Copy (2)

Kyle's Abstract - Copy - Copy - Copy - Copy (2) -...

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Exploiting Blue Light Sensitivities Through Gene Overexpression The two cryptochrome proteins found in plants, abbreviated CRY1 and CRY2, are photoreceptor plant cells for sensing blue light. Broadly, crypotochromes are partly responsible for the daily rhythms plants go though; specifically CRY1 has been shown to influence a plant’s overall biomass, leaf area, stomatal size, shape and density. The CRY2 gene is involved with circadian rhythm and flower timing. Using both wild type and transgenic tobacco seeds from Dr. James Metzger, (OSU) five cultivars of tobacco were grown including two wild types of Nicotiana sylvestris and Nicotiana tabacum and three of the species with the CRY1 gene overexpressed. Plants were grown in low, medium and high light conditions in order to test for
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Unformatted text preview: differences in irradiance acclimation through carotenoid levels, leaf surface cell density, stomatal size and shape. In line with previous work done with Arabidopsis, the CRY1 overexpressed plants show higher levels of carotenoids in both N. sylvestris and N. tabacum. Effects were most pronounced within the plants grown in low light conditions. Stomatal density and size increases also appear to be correlated to CRY1 overexpression, important for gas exchange efficiency. With the exception of mutated, N. sylvestris-S16 having a lower stomatal density than its wild type counterpart, we can conclude that CRY1 overexpression is positively correlated to carotenoid levels, stomatal density and size....
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This note was uploaded on 06/11/2011 for the course BIOCHEM 699 taught by Professor Hriton during the Fall '11 term at Ohio State.

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