DNA lab - DNA Extraction Lab Kia Salehi 309502854 Period A Partners Mani Minh Iman SBI 4U1 Teacher Ms Labi Problem Which fruit banana or strawberry will

DNA Extraction Lab Kia Salehi 309502854 Period: A Partners: Mani, Minh, Iman SBI 4U1 Teacher: Ms. Labi March 29, 2011

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Problem: Which fruit, banana or strawberry will yield the most DNA? Hypothesis: Banana will yield the most DNA. Since banana is very mushy, and much softer than strawberry, the banana cell walls will be easier to break apart than the strawberry’s, and more DNA can be extracted. Materials: 2 strawberries ½ banana Mortar and pestle 100ml water 30ml Hand soap 2 Test Tubes Graduated Cylinder 3 beakers 20ml Isopropyl 10 drops contact lens solution Inoculation loop Funnel Coffee filter paper Test tube rack Petri dish Pasteur pipette Procedures: 1. The fruit was crushed using the mortar and pestle. 2. 50ml of water was added to the fruit in the mortar. 3. The coffee filter paper was folded and placed in the funnel, and the funnel was placed in a beaker. 4. The contents of the mortar were poured into the funnel, and the fruit juices filtered into the beaker. 5. 15ml of hand soap was added to the fruit juices. 6. The fruit juices were swirled gently and were left at rest for 5 minutes. 7. The fruit juices were poured into test tube filling approximately 1/3 of the tube. 8. The test tube was tilted at a 45 degree angle and isopropyl was slowly poured in.

9. The test tube was left at rest for 3 minutes in the test tube rack. 10. DNA materials that collected at the bottom of the isopropyl layer was collected using the inoculation loop, and were put onto the petri dish. 11. Left over DNA material that could not be picked up by the inoculation loop was picked up by using the Pasteur pipette to intake the DNA material along with the surrounding isopropyl. The DNA materials were transferred to the petri dish by slowly releasing the liquid from the pipette while the petri dish was tilted at a 45 degree angle. The isopropyl pooled at the bottom corner of the dish and the DNA material remained on the dish. The pool of isopropyl at the bottom corner of the petri dish was drained by farther tilting the dish.