Enzyme Lab Temperature

Enzyme Lab Temperature - Observations: Temperature and Rate...

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Observations: Temperature and Rate of Reaction (Kia Salehi) (3 ml of H 2 0 2 used for all tests) Temperature: ( °C) Height after 5 Minutes ( mm ) Height after 10 minutes ( mm ) Average Rate of Reaction ( mm/s ) 2°C 25.0 mm 24.0 mm 6.2 x 10 –2 mm/s 8°C 17.0 mm 21.0 mm 4.6 x 10 –2 mm/s 21°C 10.0 mm 10.0 mm 2.5 x 10 –2 mm/s 37°C 20.0 mm 24.0 mm 5.3 x 10 –2 mm/s 50°C 13.0 mm 13.0 mm 3.3 x 10 –2 mm/s 60°C 2.0 mm 1.0 mm 0.42 x 10 –2 mm/s 70°C 1.0 mm 1.0 mm 0.25 x 10 –2 mm/s Size of Potato and Rate of Reaction (Fred Kim) (5% Concentration H 2 0 2 , 2 mL used for all tests) Volume ( cm 3 ) Mass ( grams) Height of Foam ( mm) Time taken to reach height ( seconds) Rate of Reaction (mm/seconds) 0.5 cm 3 1 gram 5.0 mm 388 s 0.013 mm/s 1 cm 3 2 grams 10.0 mm 116 s 0.086 mm/s 1.5 cm 3 3 grams 10.0 mm 68 s 0.147 mm/s 2.5 cm 3 5 grams 10.0 mm 44 s 0.227 mm/s Concentration of Substrate (H 2 O 2 ) and Rate of Reaction (Artem M.) *(2mL H 2 0 2 and 1mL Water for all Tests) Concentration Time Change in Ammount of Bubbles Rate of Reaction
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of H 2 0 2 (%) (seconds) Height (cm) [Height(cm)/Time(s) ] 0.5 % of H 2 0 2 0s-60.66s = 60.66seconds 1.4cm-2.0cm = 0.4 cm -very slow fizzling - slow formation of bubbles on surface 0.4cm/60.66s = 0.007 (cm/s) 1.0% of H 2 0 2 0s-60.78s = 60.78seconds 1.7cm-2.2cm = 0.51 cm - gradual fizzling -slow formation of bubbles foaming on surface 0.51cm/60.78s = 0.0084 (cm/s) 2.0 % of H 2 0 2 0s-62.00s =62.00seconds 1.6cm-2.5cm =0.9 cm -gradual fizzling of bubbles -slightly faster, still slow foaming on surface 0.9cm/62.00s =0.014 (cm/s) 2.5% of H 2 0 2 0s-60.00s =60.00seonds 1.6cm-2.5cm =0.9 cm -quick formation of bubbles on surface 0.9cm/60/00s =0.015 (cm/s) 3.0 % of H 2 0 2 0s-63.00s =63.00seconds 1.6cm-2.7cm =1.1 cm -fast formation of bubbles on surface -gradual formation continued past 1min mark 1.1cm/63.00s =0.017 (cm/s) 30.0% H 2 0 2 (EXPIRED) 0s-60.00s =60.00seconds 1.2cm-2.0cm -0.8cm - slow fizzling and formation of bubbles - H 2 0 2 a ppears to be EXPIRED , rate of RXN must be extremely fast 0.8cm/60.00s =0.133 (cm/s) (UNIMPORTANT) SOURCE OF ERROR PH and Rate of Reaction (Mani Hendesi) PH Level Time of Reaction (s) Height of Foam (mm) Rate of Reaction (mm/s) PH 2 300s 1.2 mm 0.4 x 10 –2 mm/s PH 5 300s 20.3 mm 6.7 x 10 –2 mm/s PH 7 300s 37.5 mm 13 x 10 –2 mm/s PH 9 300s 32.2 mm 11 x 10 –2 mm/s PH 10 300s 10.5 mm 3.5 x 10 –2 mm/s PH 14 300s 2.30 mm 0.76 x 10 –2 mm/s
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Surface Area and Rate of Reaction (Vitaly S.) Surface Area (cm 2 ) Number of Pieces Time (seconds) Height of foam at Time (mm) Average Rate of Reaction (mm/s) 9.79 1 T1: 150s T2: 240s H1: 15mm H2: 17mm 0.85 x 10
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Enzyme Lab Temperature - Observations: Temperature and Rate...

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