PV 92 Questions - PV 92 Questions Lesson 1 DNA Template...

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PV 92 Questions Lesson 1 DNA Template Preparation 1. Why is it necessary to chelate the metal ions out of solution during the boiling/lysis step at 100°C? In orders for the DNA to be released from the cells 2. What would happen if you did not put in the InstaGene™ matrix? The metal ions would not be trapped 2. What is needed from the cells to conduct the polymerase chain reaction (PCR)? lysosomes 3. What structures must be broken to release the DNA from a cell? The DNA template Lesson 2 PCR Amplification 1. Why is it necessary to have a primer on each side of the DNA sequence to be amplified? Complementary strand hybridization takes place when the two different primers anneal, or bind to each of their respective complementary base sequences on the template DNA. 2. How did TaqDNA polymerase acquire its name? Taq DNA polymerase extends the annealed primers by “reading” the template strand and synthesizing the complementary sequence. In this way, Taq polymerase replicates the two template DNA strands. 3. Why is TaqDNA polymerase important tool for pcr?
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This note was uploaded on 06/13/2011 for the course BIO 1500 taught by Professor Pandolfi during the Winter '08 term at Wayne State University.

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PV 92 Questions - PV 92 Questions Lesson 1 DNA Template...

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