Micro part 1

Micro part 1 - concentration of MinE is very high in the...

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concentration of MinE is very high in the middle of the cell and decreases as you move outwards Ftsz is the where the highest concentrations of MinE is Ftsz make up the main part of the divisome rod cell should be right in the middle MinE mutations with distruptive concentration – division and ring formed in the improper place When cell looks like it is elongating replication is done Ftsz ring never moves – remains until seperate Products genetically identical cells No genetic recombination, no mixing of DNA – cloning Simple budding: Cell starts to extend in one direction with a little bud Continues to enlarge, copy of DNA and pinches off Hyphae elongates with DNA inside – NOT FUNGAL hyphae Bud gets new copy of DNA and enlarges becoming more like its mother Before it leaves and officially separate it becomes flagellated When pinched off – motile- leaves Eventually loose flagellum and bud itself Microbial Growth and Ecology Macronutrients: carbon, hydrogen, oxygen, sodium, calcium, iron, etc. o Needed by the cell in large amounts so considered to be macronutrients Carbon o Needed to make organic compounds such as amino acids, fatty acids, sugars, and nitrogenous base o Lipds, nucleic acids, polysaccharides – carbon based o Makes molecules in order to grow o Fix CO2 from the atmosphere huge advantage Healthy supply of CO2 available Not a limiting molecule o If needs to depend on the organic molecule might be harder to come by – greater competition o Autotrophs use CO 2 o Heterotrophs use organic compounds
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Oxygen o Oxic environment contains oxygen o Anoxic environment lacks oxygen. o Just important as carbon Know terms on table, Obligate aerobes need oxygen to make ATP Facultative – ability to do aerobic , anaerobic, fermentation o Most versatile microorganisms on the planet o E.coli o Will prefer aerobic respiration – if oxygen is there can make more ATP Microaerophilic microbes like less than atmospheric levels of oxygen o Places that other organisms use oxygen to get levels down so they can proliferate o Aerobic respiration – just don’t want to much Aerotolerant o Tolerant of oxygen (don’t grow better if it is there just doesn’t matter)
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Don’t use it but do not care if it is there o Fermentation Obligate anaerobes – Harmful or lethal o Fermentation or anaerobic respiration o Have to grow in anoxic environments o If put in oxic environment strict obligate anaerobes o If mix facultative and obligate anaerobe both grow Reducing agent o reacts with oxygen to form water o used to remove oxygen from broth Thioglycolate broth o thioglycolate is a reducing agent o used to test an organism’s requirement for oxygen o oxygen only present at air interface In the laboratory… o Aerobes need culture medium oxygenated allow exposure to air vigorous shaking (250rpm) bubbling sterile air into the medium o Anaerobes need oxygen to be excluded from culture medium
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This note was uploaded on 06/13/2011 for the course BIOL 2051 taught by Professor Brininstool during the Spring '07 term at LSU.

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Micro part 1 - concentration of MinE is very high in the...

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