Chapter 25 Testbank

Chapter 25 Testbank - BCMB 3100 - Chapter 20 DNA Replication

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Chapter 25 DNA Metabolism Multiple Choice Questions 1. DNA replication Page: 977 Difficulty: 2 Ans: C The Meselson-Stahl experiment established that: A) DNA polymerase has a crucial role in DNA synthesis. B) DNA synthesis in E. coli proceeds by a conservative mechanism. C) DNA synthesis in E. coli proceeds by a semiconservative mechanism. D) DNA synthesis requires dATP, dCTP, dGTP, and dTTP. E) newly synthesized DNA in E. coli has a different base composition than the preexisting DNA. 2. DNA replication Page: 978 Difficulty: 2 Ans: D When a DNA molecule is described as replicating bidirectionally, that means that it has two: A) chains. B) independently replicating segment. C) origins. D) replication forks. E) termination points. 3. DNA replication Page: 979 Difficulty: 2 Ans: D An Okazaki fragment is a: A) fragment of DNA resulting from endonuclease action. B) fragment of RNA that is a subunit of the 30S ribosome. C) piece of DNA that is synthesized in the 3 ' 5 ' direction. D) segment of DNA that is an intermediate in the synthesis of the lagging strand. E) segment of mRNA synthesized by RNA polymerase. 4. DNA replication Pages: 979-984 Difficulty: 2 Ans: C Which one of the following statements about enzymes that interact with DNA is true? A) E. coli DNA polymerase I is unusual in that it possesses only a 5 ' 3 ' exonucleolytic activity. B) Endonucleases degrade circular but not linear DNA molecules. C) Exonucleases degrade DNA at a free end. D) Many DNA polymerases have a proofreading 5 ' 3 ' exonuclease. E) Primases synthesize a short stretch of DNA to prime further synthesis.
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Chapter 25 DNA Metabolism 5. DNA replication Page: 982 Difficulty: 2 Ans: C E. coli DNA polymerase III: A) can initiate replication without a primer. B) is efficient at nick translation. C) is the principal DNA polymerase in chromosomal DNA replication. D) represents over 90% of the DNA polymerase activity in E. coli cells. E) requires a free 5 ' -hydroxyl group as a primer. 6. DNA replication Pages: 981-982 Difficulty: 3 Ans: E The proofreading function of DNA polymerase involves all of the following except: A) a 3 ' 5 ' exonuclease. B) base pairing. C) detection of mismatched base pairs. D) phosphodiester bond hydrolysis. E) reversal of the polymerization reaction. 7. DNA replication Page: 982 Difficulty: 2 Ans: D The 5 ' 3 ' exonuclease activity of E. coli DNA polymerase I is involved in: A) formation of a nick at the DNA replication origin. B) formation of Okazaki fragments. C) proofreading of the replication process. D) removal of RNA primers by nick translation. E) sealing of nicks by ligase action. 8. DNA replication Pages: 982-983 Difficulty: 2 Ans: C Prokaryotic DNA polymerase III: A) contains a 5 ' 3 ' proofreading activity to improve the fidelity of replication. B)
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This note was uploaded on 06/14/2011 for the course CHEM 500 taught by Professor Sureus during the Spring '11 term at Wisconsin Milwaukee.

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Chapter 25 Testbank - BCMB 3100 - Chapter 20 DNA Replication

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