10_Processing

Lehninger Principles of Biochemistry

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Lecture 10 RNA Processing Capping Splicing RNA Tails Key topics :
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Reading 26 | pp. 1021-1033 © 2009 W. H. Freeman and Company
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RNA Processing Almost all newly synthesized RNA molecules (primary transcripts) are processed to some degree in eukaryotic cells The 5’-end is capped w/ methylguanosine Introns are spliced out Poly-A tail is built at the 3’ end Processing is catalyzed by protein-based enzymes and by RNA-based enzymes ( ribozymes ) Only some prokaryotes have to splice out introns but many process their tRNA precursors
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The 5’ RNA cap Structure
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The capping reactions Capping protects mRNA from 5’exonuclease degradation
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Synthesis of the cap is carried out by enzymes tethered to the CTD of Pol II. The cap remains tethered to the CTD through an association with the cap-binding complex (CBC)
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Formation of Primary Transcript and Its Processing in Eukaryotes The removal of introns from the primary transcript is called splicing
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Humans have lots of introns
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10_Processing - Lecture 10 RNA Processing Key topics...

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