16_DNA Tech I

Lehninger Principles of Biochemistry

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Unformatted text preview: Lecture 16 DNA Technologies I DNA analysis methods DNA cloning techniques PCR Site directed mutagenesis Key topics : Reading 9 | pp. 303-317 2009 W. H. Freeman and Company Recombinant DNA Artificially created DNA that combines sequences that do not occur together in the nature Basis of much of the modern molecular biology Molecular cloning of genes Over-expression of proteins Transgenic food, animals Separation of DNA by Electrophoresis Negatively charged DNA migrates to the anode in the presence of an electric field Agarose gel hinders the mobility of DNA molecules Mobility depends on the size and the shape Small molecules faster Compact molecules faster Practical use is DNA analysis DNA purification DNA-protein interaction studies Agarose Gel Electrophoresis Agarose Gel Electrophoresis DNA Cloning Organism cloning: Creation of identical copies of an organism DNA cloning: Creation of identical copies of a piece of DNA (gene) isolate a specific gene from the source organism and amplify it in...
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16_DNA Tech I - Lecture 16 DNA Technologies I DNA analysis...

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