21_Biosignalling1

Lehninger Principles of Biochemistry

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Lecture 21 Biosignaling 1 General features of signal transduction Structure and function of G-protein coupled receptors Structure and function of enzyme-linked receptors Structure and function of gated ion channels Key topics :
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Reading 12 | 419-444 © 2009 W. H. Freeman and Company
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Biological Role of Signal Transduction Cells receive signals from the environment beyond the plasma membrane Antigens Hormones Neurotransmitters Light Touch Pheromones These signals cause change in the cell’s composition and function Differentiation and antibody production Growth in size or strength Sexual vs. asexual cell division
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Receptors Receptor: A membrane-bound or soluble protein or protein complex, which exerts a physiological effect (intrinsic effect), after binding its natural ligand. G-protein coupled receptors Epinephrine receptor Enzyme linked receptors Insulin receptor Ligand-gated ion channels Nicotinic acetylcholine receptor Other membrane receptors Integrin receptors Nuclear receptors Steroid receptors
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Four Features of Signal- Transducing Systems
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Receptors Bind Specific Ligands Typical ligands are Small ions ferric ion: bacterial ferric receptor Organic molecules Adrenalin: epinephrine receptor Polysaccharides Heparin: fibroblast growth factor Peptides Insulin: insulin receptor Proteins Vascular endothelial growth factor: VEGF receptor
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Receptor Binding Studies: Filter Assay Rationale: Equilibrium binding of labeled ligand with the receptor R + L RL The bound complex becomes radioactive Free receptor remains non-radioactive Free ligand can pass through the filter Complex cannot pass because the protein binds to the filter Steps: Isolate membranes
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21_Biosignalling1 - Lecture 21 Biosignaling 1 Key topics...

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