bacteriology - Bacteriology Exam Question and Answers Holla...

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Bacteriology Exam Question and Answers. Holla. EXAM I: 1) What are 3 benefits of being small? -rapid nutrient exchange – large surface to volume ratio. -rapid growth -large populations able to be formed -mutations can lead to adaptability 2) What are some features of an endospore that contribute to its resistance to heat, radiation, and chemicals? -SASP's (small acid-soluble proteins) – bind tightly to DNA in core and protect it from potential UV radiation, desiccation, and dry heat. -calcium-dipicolinic acid complex (fig. 4.42) reduces water availability within the endospore, helping to dehydrate it, which increases its heat resistance. -exosporium and spore coats form around endospore. 3) What are the 6 characteristics of cellular life? -Compartmentalization/Metabolism -Reproduction -Differentiation -Communication -Movement -Evolution 4) describe how chemoheterotrophs establish and maintain a proton motive force (PMF) and generate ATP. Include a drawing in which key components are labeled. What is this process called? What would happen if the cytoplasmic membrane is damaged? -ATP generated by oxidative phosphorylation and substrate level phosphorylation. (oxidation, reduction, then regeneration of NAD+ and FAD+. -process called oxidative phosphorylation. EXTERIOR H+ H+ ATP Synthase FAHD2 NADH The FADH2 and NADH donate electrons (to the blue circles) and then move from circle to circle. CYTOSOL OH- OH- -If the membrane was damaged, the PMF would be disrupted and no ATP would form. ADP+Pi ATP 5) Discuss Pasteur's experiment for disproving spontaneous generation. What was convincing about it? -Swan neck sterile tube with sterile broth; allowed dust to collect in tube; disproved spontaneous generation and that growth came from dust (microbes) in the air. 6) Discuss diversity of photosynthetic prokaryotes. Include discussion on phylogenics (ie, group/phylum names), physiology, and at a molecular level. -cyanobacteria (oxygenic), green sulfur bacteria (anoxygenic), green non-sulfur bacteria (anoxygenic), purple sulfur bacteria (anoxygenic), purple non-sulfur bacteria (anoxygenic), prochlorophytes (oxygenic)
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anoxygenic: H2S is electron donor, bacteriochloroplast A and B oxygenic: H2O goes to ½ O2 + 2H+ I can't read my handwriting, so youse are on your own with this one. 7) Design an experiment to determine a possible pathogen. (remember Koch's postulates.) -just use Koch's postulates. Grow suspected pathogen from diseased animal in lab culture, inject healthy animal with suspected pathogen, watch the healthy animal helplessly die from pathogen. Now, you have determined a possible pathogen. Go wash the blood of the innocent from your hands. 8) Explain how prokaryotes are classified by their energy source and carbon source. Chemoorganotrophs: glucose + O2 goes to CO2 + H2O (aerobic vs anaerobic, fermentation)
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This note was uploaded on 06/17/2011 for the course BIOL 550 taught by Professor Long during the Fall '10 term at South Carolina.

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bacteriology - Bacteriology Exam Question and Answers Holla...

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