BIOL550 Lecture M2_01&02-1

BIOL550 Lecture M2_01&02-1 - compatible solutes...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture Mod2_01 & 02 Microbial growth and nutrition Growth of cell vs population Binary fission Clonal reproduction “Sweet Spot” = Niche (Fundamental) Energy Source Nutrients Environmental conditions Nutrients Macronutrients Carbon Nitrogen Others: P, S K Mg, Ca Trace elements (micronutrients) Inorganics e.g. Iron Chelators Siderophores Growth factors Organics Precursors and cofactors e.g. vitamins and amino acids Working with bacteria in laboratory setting Cultivation (culturing) Culture media Nutrient vs minimal media Define vs complex media Categories General media Enrichment media Selective media Differential media Forms Broth Agar Plates Slants Motility Other means of cultivating microbes
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Environment Temperature Cardinal temperatures Properties affected pH Salinity Osmolarity Water activity
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: compatible solutes Oxygen Aerobes vs anaerobes (further categorized) Toxic oxygen forms Adapations Culturing anaerobes Pressure Bacterial growth Reproduction Individual vs population Individual reproduction Clonal Binary Fission FtsZ ring Bactoprenol Population dynamics Generations Generation time Batch Cultures Conditions Growth curve Lag Exponential Stationary Death Chemostat culture Steady state Wash out vs. . batch cultures Measuring bacterial Abundance Direct methods Light microscopy Epifluorescent microscopy Flow cytomety Indirect methods Turbidity of a culture Dry weight Viability Plate counts & Colony forming units (CFU) Measuring bacterial growth Frequency of dividing cells Metabolic activity...
View Full Document

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern