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quiz 9 - MCB 200 Name Fall 2007 Quiz#9 Section 1 1 4 1 1 5...

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Unformatted text preview: MCB 200 Name Fall 2007 Quiz #9 Section +1 +1 : +/ 4. +1 +1 5. +/ 6. 5kb What term describes the type of molecule produced when segments of DNA from two different sources are combined to form one continuous sequence? F€Cor17bi WQWZL: DNA What enzyme is used to covalently join complementary DNA segments? D NA I) (1“ 56. What enzyme is capable of synthesmng complementary DNA from a Single- stranded RNA template? reverse frat r7507}; $066 What type of circular DNA molecule derived from bacterial cells is capable of replicating independently of the host genome? Plasrfifll DNA How does cDNA differ from the corresponding genomic coding sequence? CDMA 0/085 [70% conform lfif/‘ofif What property of a DNA vector allows for selective growth of transformed cells? /‘ N \ ~-. (INDIA; Dir C F85! S'éfiflCEgefle The 11 kb circular molecule shown below to the left has restriction enzyme sites for BamHI and EcoRI. In the diagram of the gel shown below to the right, draw the DNA bands you would expect to visualize, after running electrophoresis, following digestion with BamHI alone (lane 1), EcoRI alone (lane 2), and BamHI and ECORI together (lane 3). For clarity, please also label the expected band sizes on the gel for each of the digests. The dashed lines on the right of the gel indicate a DNA ladder of sizes 1-12 kb. 1 2 3 ladder BamHl 3 kb 12 kb 9 kb 6kb 3kb 1kb ...
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