Unformatted text preview: MCB 200 Name
Fall 2007 Quiz #9 Section +1
+/ 6. 5kb What term describes the type of molecule produced when segments of DNA from
two different sources are combined to form one continuous sequence? F€Cor17bi WQWZL: DNA What enzyme is used to covalently join complementary DNA segments? D NA I) (1“ 56.
What enzyme is capable of synthesmng complementary DNA from a Single-
stranded RNA template? reverse frat r7507}; $066 What type of circular DNA molecule derived from bacterial cells is capable of
replicating independently of the host genome? Plasrﬁﬂl DNA How does cDNA differ from the corresponding genomic coding sequence? CDMA 0/085 [70% conform lﬁf/‘oﬁf What property of a DNA vector allows for selective growth of transformed cells?
/‘ N \ ~-.
(INDIA; Dir C F85! S'éﬁﬂCEgeﬂe The 11 kb circular molecule shown below to the left has restriction enzyme sites
for BamHI and EcoRI. In the diagram of the gel shown below to the right, draw
the DNA bands you would expect to visualize, after running electrophoresis,
following digestion with BamHI alone (lane 1), EcoRI alone (lane 2), and BamHI
and ECORI together (lane 3). For clarity, please also label the expected band
sizes on the gel for each of the digests. The dashed lines on the right of the gel
indicate a DNA ladder of sizes 1-12 kb. 1 2 3 ladder BamHl 3 kb 12 kb
9 kb 6kb 3kb 1kb ...
View Full Document
- Spring '08