BBlecture5S10s

BBlecture5S10s - 5.1 Microbial Nutrition 5.2 Culture Media...

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Unformatted text preview: 5.1: Microbial Nutrition 5.2: Culture Media 5.3: Laboratory Culture of Microorganisms Nutritional Needs & Culturing of Microbes nut 1 overview of cell metabolism: • catabolic : energy releasing • anabolic : energy requiring macronutrients include: C, N, P, S, K, Ca, Mg, Fe micronutrients include: trace elements, growth factors nut 2 phototrophs: light as energy source chemotrophs: chemicals as energy source • chemoorganotrophs : organic chemicals • chemolithotrophs: inorganic chemicals recall: energy classes of microorganisms our focus: • most commonly encountered in lab studies • important in nature • important in disease • possible to grow members of other classes using same general principles – though different nutrient materials may be supplied nut 3 Culture Media defined : made from purified chemicals e.g., glucose-mineral salts broth complex (undefined) : exact chemical composition not known e.g., YE (yeast extract) broth • agar – polysaccharide derived from algae • forms a gel at temperatures of interest, including 37ºC • not many microorganisms can degrade it • widely used as solidifying agent in microbial media e.g., glucose mineral salts agar, YE agar, nutrient agar nut 4 macronutrients : required in large amounts nut 5 micronutrients : • required in lesser, sometimes trace amounts • not every element is required by all cells nut 6 growth factors : • organic compounds required in small amounts • not every growth factor is required by all cells nut 7 defined undefined nut 8 defined Serratia marcescens...
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This note was uploaded on 06/18/2011 for the course BIO 150 taught by Professor Butler during the Spring '11 term at Waterloo.

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BBlecture5S10s - 5.1 Microbial Nutrition 5.2 Culture Media...

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