BBlecture5S10s - Nutritional Needs Culturing of Microbes...

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5.1: Microbial Nutrition 5.2: Culture Media 5.3: Laboratory Culture of Microorganisms Nutritional Needs & Culturing of Microbes nut 1
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overview of cell metabolism: catabolic : energy releasing anabolic : energy requiring macronutrients include: C, N, P, S, K, Ca, Mg, Fe micronutrients include: trace elements, growth factors nut 2
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phototrophs: light as energy source chemotrophs: chemicals as energy source chemoorganotrophs : organic chemicals chemolithotrophs: inorganic chemicals recall: energy classes of microorganisms our focus: most commonly encountered in lab studies important in nature important in disease possible to grow members of other classes using same general principles – though different nutrient materials may be supplied nut 3
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Culture Media defined : made from purified chemicals e.g., glucose-mineral salts broth complex (undefined) : exact chemical composition not known e.g., YE (yeast extract) broth agar – polysaccharide derived from algae forms a gel at temperatures of interest, including 37ºC not many microorganisms can degrade it widely used as solidifying agent in microbial media e.g., glucose mineral salts agar, YE agar, nutrient agar nut 4
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macronutrients : required in large amounts nut 5
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micronutrients : required in lesser, sometimes trace amounts not every element is required by all cells nut 6
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growth factors : organic compounds required in small amounts not every growth factor is required by all cells nut 7
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defined undefined nut 8 defined
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