BBlecture10S10s

BBlecture10S10s - Principles of Bacterial Genetics 11.9:...

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Principles of Bacterial Genetics (genetic exchange in prokaryotes) 11.9: Genetic Recombination 11.10: Genetic Transformation 11.11: Transduction 11.12: Conjugation: Essential Features 11.13: The Formation of Hfr Strains and Chromosome Mobilization 11.14: Complementation 11.15: Gene Transfer in Archaea 11.16: Mobile DNA: Transposable Elements gex 1
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genetic recombination : SSB – single stranded binding protein resolution involves nuclease and DNA ligase activities RecA -like proteins found in all prokaryotes, yeast, higher eukaroytes examined (Fig 11.13, 12th ed) gex 2
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chi form i somerize a b b a A A B B resolution patch resolution b a A B A b B a exchange of flanking markers (splices) A B a b you are not responsible for this!!! gex2 a
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DNA transfer : transformation : transfer of bacterial genes involving free DNA transduction : transfer of host genes from one host to another by a virus conjugation : transfer of genes from one prokaryotic cell to another by mechanism involving cell-to-cell contact and a plasmid (Fig 11.12, 12th ed) gex 3
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search for a rare genetic recombinant amongst nonrecombinants is based on selectable markers : (Fig 11.14, 12th ed) gex 4
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This note was uploaded on 06/18/2011 for the course BIO 150 taught by Professor Butler during the Spring '11 term at Waterloo.

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BBlecture10S10s - Principles of Bacterial Genetics 11.9:...

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