ch369_sp11_class_5_notes

ch369_sp11_class_5_notes - Todays plan Finish with chapter...

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Today’s plan - Finish with chapter 3, “Genes to Proteins”. Then, on to chapter 4, “Proteins”.
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When DNA is sequenced using a mixture of dNTPs & ddNTPs, which of the following is true of the new DNA strand that has been made in the sequencing reaction? Clicker question.
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Absorbance of UV light by nucleic acids increases by about 30% when the base pairs are melted: From a melting curve such as above, it is possible to determine the “ melting temperature ” of nucleic acids. (In the graph above, it looks like the DNA melts at about 40 deg C) Detecting “melting” of DNA by change in absorbance of UV light. Absorbance of UV light at 260 nm Temperature (deg C) melting temp.
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Beer’s law: The change in UV absorbance due to a change in base stacking is called hypochromicity ”.
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“PCR” is used to make many copies of a DNA sequence. So if you have just a little bit of DNA, you can use PCR to make many identical copies (and get enough DNA for sequence analysis). Another tool of nucleic acid chemistry: “Polymerase chain reaction” or PCR.
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PCR is used to make many copies of DNA.
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What things do you need to do PCR? DNA template (to be copied). dNTPs (mixed dATP, dCTP, dGTP, dTTP) DNA polymerase (preferable thermostable, so it can stand the heating and cooling cycles) A good DNA polymerase for use in PCR is stable at high temperature: DNA pol from Thermus aquaticus (“Taq polyerase”) DNA pol from Pyrococcus furiosus (“Pfu polymerase”, from a thermophilic archaea found near Vulcano Island, Italy)
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(figure from chapter 3)
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(Chapter 4 of textbook) Some biological functions of proteins: Enzymes - proteins that catalyze chemical reactions. Transport proteins
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ch369_sp11_class_5_notes - Todays plan Finish with chapter...

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