ch369_sp11_enz_3_notes - Which statements are true about...

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Unformatted text preview: Which statements are true about the tetrahedral intermediate that forms when trypsin attacks a peptide bond? Choose one best answer. Plan for today is to finish chapter 7. “Enzyme kinetics & enzyme inhibition” Last time - Michaelis-Menten enzyme kinetics: [S] = initial substrate concentration, typically in units of moles/liter. v o = initial reaction velocity (such as mM of product produced / sec) K M is the substrate concentration when the initial reaction Three numbers that can describe an enzyme’s catalytic abilities are: K M and V max and k cat initial reaction velocity V [S] Another quantity used to characterize enzymes is k cat . k cat = V max / [E] tot al k cat is called “catalytic constant” or “turnover number”. k cat = number of catalytic cycles by each active site per second. Enzyme k cat Staph. nuclease 95 sec-1 Chymotrypsin 190 sec-1 Trios phosphate isomerase 4300 sec-1 Carbonic anhydrase 1,000,000 sec-1 Finding V max and K M : May use a “ Lineweaver-Burk plot ” * Lineweaver-Burk plot for an enzyme catalyzed reaction is shown. What is K M for this enzyme?...
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This note was uploaded on 06/20/2011 for the course CHEM 369 taught by Professor Hoffman during the Spring '11 term at University of Texas at Austin.

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ch369_sp11_enz_3_notes - Which statements are true about...

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