Bio informatics #3 - sequence like a cell would. 2) What...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Bioinformatics Assignment #3 3/6/11 1) Explain why the computer translation of an mRNA sequence can result in six different possible protein sequences. When the computer translates the mRNA sequence, six different possible protein structures are given because the program doesn’t know which of the two original DNA strands should be translated for the RNA. The program can start translating at the first base, which will give the bases for one direction. However, the program could also start at the second or third nucleotide, creating a translation beginning from the chosen starting point. Since the process could happen at either strand, there are six ways that the program can translate a given mRNA sequence. Basically, this computer program does not receive biological symbols to guide its translation, and therefore does not translate a mRNA
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: sequence like a cell would. 2) What feature(s) helped you decide which possible translation frame was correct? We decided our possible frame because there was a methionine at the beginning, a chain of amino acids, then a stop. 3) Comparing the translated products of the normal and mutant PAH mRNA's, and the PAH protein reference sequence, which are the same as each other? Which one is different from the others? Note the specific amino acid change. Use both the single letter amino acid code and the three letter amino acid code, and give the position number of the change. The translation of the normal PAH mRNA and the reference sequence for PAH protein are the same as each other. The translation of the mutant PAH mRNA sequence is different because at the 280 th amino acid, the E (glu) changes to a K (lys)....
View Full Document

This note was uploaded on 06/20/2011 for the course BIO 2960 taught by Professor Staff during the Spring '08 term at Washington University in St. Louis.

Ask a homework question - tutors are online