Name_________
KEY
___________________________
Section 12:002:00pm / 2:304:30pm
MIC 302, Spring 2011
Homework #1
(20 points)
TURN HOMEWORK IN AT THE BEGINNING OF CLASS ON MONDAY, JANUARY 24
SHOW ALL WORK AND USE CORRECT SCIENTIFIC NOTATION AND
SIGNIFICANT FIGURES
Solution Problems
:
Calculate the amount of reagent and water required to make the
following solutions (2 points each):
1.
1.50L of 1.00M NaCl:
(1.5L)(1mol/L)(58.4g NaCl/mol) =
87.6 g NaCl + H
2
O up to 1.50 L
2.
250mL of 20% glycerol:
(0.20)(250mL)=
50g (or mL) glycerol +
H
2
O up to 250mL
3.
15mL of 2.5M MgCl
2
:
(0.015L)(2.5mol/L)(95.2g MgCl
2
/mol)=
3.6g MgCl
2
+ H
2
O up to 15 mL
4.
0.25L of 10.0mM CaCl
2
:
(0.25L)(0.010mol/L)(110.98g CaCl
2
/mol)=
0.28g CaCl
2
+ H
2
O up to 0.25L
5.
50.0mL of 20.0% Arabinose:
(0.20)(50.0mL)=
10.0g (or mL) arabinose + H
2
O up to 50.0mL
This preview has intentionally blurred sections. Sign up to view the full version.
View Full Document
Dilution Problems
:
Design a
simple
(least number of steps) dilution scheme to get a
statistically significant
number of colonies on your TSA plate.
Be sure to draw out your
dilution scheme, include the
(1)
volume of dilution blanks used,
(2)
volumes transferred,
(3)
the fold dilution that corresponds to the volume transferred, and finally
This is the end of the preview.
Sign up
to
access the rest of the document.
 Spring '08
 STOUT
 TSA, TSA plate, dilution scheme

Click to edit the document details