Answers Assignment 3

Answers Assignment 3 - BIS 104, Section 1, Spring quarter...

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BIS 104, Section 1, Spring quarter 2011 Assignment 3, 5/17/2011 Note: This assignment is for your own benefit – you are not asked to hand it in. Answers to this assignment will be posted at a later date. 1. A patch of membrane containing β -adrenergic receptors and other relevant membrane proteins was pulled from a cell using a glass pipette, similar to the process used in a patch clamping technique. As shown in the diagram below, the external surface of the membrane is in contact with the solution in the pipette and the cytoplasmic surface of the membrane is immersed in a small well. The well contains buffer that is equivalent to the salt composition (Mg ++ , Ca ++ , etc.) of the cytosol and contains ATP, but does not contain other small molecules such as other nucleotides. intact cell pipette - - well outer solution inner solution pipette - Various substances were added to either the solution in the bore of the pipette (outer solution) or to the solution in the buffered well (inner solution). The solution in the well (inner
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Answers Assignment 3 - BIS 104, Section 1, Spring quarter...

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