Key to Midterm

Key to Midterm - NAME: _ BIOLOGICAL SCIENCES 104, SECTION 1...

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NAME: ______________________ BIOLOGICAL SCIENCES 104, SECTION 1 MIDTERM EXAMINATION May 3, 2011 Marilynn E. Etzler Students, Answers are provided in red for each question on the following pages. If you look over this exam you will see that the information required to answer each question was given in the lectures to this class. You will also see that some questions were very similar to previous questions from your quiz or out of class assignments. A number of you were able to answer the easier questions, but when it came to having to apply the material you learned to a more analytical question you could not do it. This should be a wakeup call to you that you cannot get a good grade in this class by simple memorization and instead must settle down and try to understand the material. Except for some of you that got very low scores, there is still time to improve your grade in the future, but you must take action now. If you wait until the week before the final, there will be little that can be done to help you. The final exam for this class will be comprehensive covering all of the material we have covered in this class. Marilynn Etzler
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2 NAME: ______________________ 1. Several techniques that have been employed in studies of cell biology are listed below. A. Patch clamp G. Use of artificial membranes B. SDS gel electrophoresis H. Fluorescence recovery after photobleaching C. Electron microscopy I. Sedimentation equilibrium gradient centrifugation D. Light microscopy J. In vitro reconstitution assays E. Immunoblot assay K. Use of Langmuir trough F. Pulse chase assay L. Use of black membranes Please use the letters beside each of the above techniques in answering the following questions. Only use one letter for each question. If you feel that more than one letter can be used, choose the answer that is the most appropriate. a) (2 points) The oldest technique in cell biology. _____ D _____________ b) (2 points) A technique with which one can determine the diffusion coefficient of a protein in a membrane. _______ H ______________ c) (2 points) A technique that could be used to separate smooth endoplasmic reticulum from rough endoplasmic reticulum. _______ I ____________ d) (2 points) A technique that was used to determine the function of the signal recognition particle. ______ J ________________ e) (2 points) A technique that was used to establish that lipids form bilayers in membranes. ______ K ________________ f) (2 points) A technique used to follow the route of transit of a protein through the secretory system. ______ F _________________
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3 NAME: ______________________ 2. Each of the organellar fractions below is labeled with a letter. Please use these letters in answering the questions below. No partial credit will be given for this set of questions, so please be exact in answering them. A.
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This note was uploaded on 06/21/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.

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Key to Midterm - NAME: _ BIOLOGICAL SCIENCES 104, SECTION 1...

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