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lect30sample - large inserts cosmid lambda genomic 2...

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1. antibody probe vector? library? general approach? 2. chromosomal position plus nearby clone vector? library? general approach? Always ask, how will you detect clone of Always ask, how will you detect clone of yfg ? If you ? If you are given: are given: Answers on next page.
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1. antibody probe vector? expression library? cDNA general approach? Make cDNA library in expression vector, transform bacteria, select transformants, make filters & screen with Ab.
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Unformatted text preview: large inserts - cosmid, lambda genomic 2. chromosomal position plus nearby clone vector? library? general approach? Make genomic library in lambda or cosmid, transform bacteria, select transformants, make filters & screen with clone. Repeat until find yfg . Always ask, how will you detect clone of Always ask, how will you detect clone of ? If you ? If you are given: are given:...
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