KeyII-Spring 07

KeyII-Spring 07 - / L3122007 Drummond Exam ll \g ‘l. (10...

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Unformatted text preview: / L3122007 Drummond Exam ll \g ‘l. (10 points) Draw a membrane with an embedded receptor tyrosine kinase that is anatomically correct, including any cofactors that might be there. Identify a series of steps that would convert ligand binding to changes within the cell. Your answer should show any intracellular cascade that might beimportant for thin 5 like hosphorylatingtranscri tion factors to alter events in the nucleus} m . (Wales/“1h ——.—“I\l liafiv‘l ‘d'x/wplAs V um 90"!“ 4w, {27kg +9 [What Crass ~fimsfhut/ (alt (ac! ‘ a") W" wow ~5~w W‘fil’uflj' “w 27K train}; k/fflfijfll %M [’14 qunscrzptwa {Mien s— Sun N51,,Io/lan ,gv\ ppgl‘i’“ /N[“C”l Will 4/4” Btu, ewazfimy ovum: mule“! gwwl/ US 69% 7 N m W » HM ' x % r—fi MM)'kl/WSL—k1/HSL « mt ‘ , PM «at, ’7 a ‘ A“, lividwi “man” “I”; V” JCADP @ 3 RN ¢ RM prom. r in t «animal... 4 v P’ ° “"’"“*’ ». a!“ 9'(-\~/¢.}\ y! 41 A @ « l a / 1"“ MN “MW JConv C” x 3 i _i c ’m ,7! art—v“ 44.... mm mm m m W “2. (10 points). You know that the enzymes of the citric acid cycle are found within the m“) \ mitochondrial matrix. Draw a diagram that shows where these proteins are translated and how “to; ‘ they make it inside the mitochondria. —/ (7+0?) I C Mth _ Mikel/“Mir”, 3:7)9/“0 1 Vhlflsiahw‘ Mn"; ’ I H 4/ l" (74075} a mulnx “Jilinan $3qu S‘fiw ALE Aw d‘mg I k: A". . v M401 ’3 pMk/MIM‘I (AA Luv” 4” 0‘ "((tpfil U! 4W ovule Ml’LOCIAInJ'HH’ KAI-«Abrowa "[Lu‘ ‘V-l’u. tum at! 41“ 411W 4mwluflgw‘ in,” c J M ‘I r u 0““: Hum“! “Shkl AA P 1 494 WWW ‘UA 0 5‘4 4W. Pnu Sigma: (1 clemasz “LP i “AA fl”. P/‘U‘LttK (J fiwal lo {0U / ‘ 3:: 4w pm Hi -S jwfld WW 1 u ‘ . 6"“ Ckkpqrms b7 d’wV‘fi'n ‘L F re «Lu/\ J L312 2007 Drummond Exam II AVG-“h Your name 3. (10 points). Draw a diagram that s ows how a protein with a nuclear import sequence is transported in the nucleus. Make su and at least in ne place gives some i your diagram accurately depicts the nuclear membrane a what the nuclear pore looks like. Remember any cofactors or e ergy sources required, t 0! Dimensions here are important. N fifiN’rmfi U, /—\_/-) git Flo is.ch frfnlfu“ 0" ((crplar mam am wt 0» 4_____,__/' l0“) lul an“ r16 9? ku- __ yr u Liar Jmaspori “mph, loud: +9 515ml Jrgurucan our-'14..“ {or Meir“ :warJ — Vii/‘14 Justin H 'fi’lrrvjh —IM Furt For-AC: lnjnoL-f fifiN+ mp WWW“ Lu. N - 4 F 415m 9' {Mg firflhud mi” ’H" "\L‘tlrw ' “W 1924M+LTP __ hkfihfir 'fwwpart CaMpith wont) Owlfit "Hmt Inuit l'fuJ RAM; 6:”) hydrnly-llj ‘an “Hurt rrrhut' 4? .P’Wl chzplr‘ L‘ 4. (4 points) Sketch a kinesin motor protein. identifying and carefully labeling all the important features. V7.“ 5/” Lil/hi (Lin-rm} [at [(61 ’09” S-lrm ohm , mu 0'{ New); CJ/wuuurIt KG:be mono wh‘glq Cal/‘59 :togie $5“ [Mn/L and?) which bum? nacho-Putt! Phlip‘mafl ti. 1”“ MLN‘ILJH’ILJ' L312 2007 Your name Exam ll Drummond 5. (7 points) A match made in heavent Pick the answer from the following list that BEST describes the mechanism of action of each of the following proteins or small molecules. Some answers are used more than once—just write the right letter next to the number. Please also note that most answers apply only to one polymer—go with the combination that fits for each case. The polymer in each case is either actin or microtubules. and the monomer is either the actin protein or am tubulin. A. Binds to the filament (polymer) and prevents elongation 1 B. Binds to the filament and prevents collapse 'II C. Binds to the monomer and prevents elongation 'I D. Binds to the monomer and is required for elongation 'I E. Binds to the polymer and digests it - J E 1. Gelsolin #- B 2. Taxol - Err-ruin“ dryer -mgr; D Q 3. ATP 1) 4_ (311:: 6-.- (ath ti. app JthJu'Lh rural. C 5. Cytochalasin Mr _ B B. Jasplakinolides C 7. Colchicine _ lbw“ WW Mr, 9., 6. (9 points) Identify the intracellular space to which each of following proteins would be targeted. Some information that you need to know might be missing in some cases, due to a lack of space. Still, make your best assessment in each case. If you feel that you need to explain your answer, do so in the space just below the sequence. Seguence: intracellular sgace ...AlaHisGluAlaGlyArgAT’gVaIAsplPheAlathGlyProSerThrCOO'... C {I 05“ " k D E L ...AlaHisGluAtaGlyArgArgValAspPheAlaGlyLysAspGluLeuCOO'... raimlm m ER lwkm t .t 1 + k D E 'r _ ...AlaHisGluAlaLysArgAFgArgLysValPheAlaGlyLysAspGluLeuCOO'... hmlw) g M'Hlv‘l‘fron mm? lam HaN-GlyAlaLYsAlaCysAlaLysGlu LeuValATrgAspGlyPheAlaKi'gGly... lm :l-vthand run. HaN-GlyAlaHisCysAlaLysGluLeuValAspAspGlyPheAlaGlyGlyPro... (~5li 1 H3N-GlyzfitlaLeuliauAlaVallleLeuValPheAlaLeuPheAlaGlyéspPro... 5K “yd-r'flpkfihu. / L312 2007 Drummond Exam ll Your name 7. (10 points) Proteins that are targeted for lysosomes do not enter them directly. instead, the Golgi apparatus feeds the lysosome via transport vesicles. Draw a diagram that shows how a nuclease. for example, might be captured at the Golgi and selectively move to the lysosome. Be sure to indicate the size of the transport vesicle. Mg“ lam-t! L“ D‘ |' “find afllfi" Marti” "‘ L“ ' I bani} H al‘phfif C / aw" was cumw V5 51 LL; ‘f'b my»; v4 0 o t.sNAeE l'fEDSO‘flf-v amt» M"! M “mob! l w‘“ firs...“ at HMRH V” SNM F in {out d 9— mamah FiMEO-i a? 4M. Wicf'k'tlrrmu _ , . . 8. (10 pomts) draw a diagram that shows how a protein With two transmembrane helices is inserted into the ER (endoplasmic reticulum) membrane. Be sure to label any key features essential to the translated protein or the apparatus required to carry out the process. (705:5 a.“ \’ ’ .1 i; 6/ ‘3 . a . KEN“. a X _ Y } F» (N I”? J 1r“) ;_'.- “ LL)“ ‘3 I-Q V35 ‘ j m L— L.- MEN .4 _ 3mm“; (qua-i.th finial: Lin-U _ JIM 5?? NHer in hjdf‘flphfl'ytfl tip-Lth 4 “W; #MMl‘hn _. ‘HV— h7dl‘np‘mlflt Shirl lrnMfi-r Jrqwm . “l‘r N -1ti’mm-j (41 V” “M imml'ujwq PM “Mam; IO fume m h \ D 'K tn Ii 'llna r '3 10.: 41M 4PM;in W“. {’ _""\\ i P’Vl‘hn M4“ ‘H-u. Ea ‘ '. «HM Ea Mani-rant l-mr in”? Cm” be _ (finial wl Initial S—iml ~ifiiviaFIr {Lfimfntt will smut-III:i on Mal-Erm.ql Ink”! 0'41, ptt-[tc‘ijin a! 14m r’f—fis" D / \ ff“: 3 at 6—“- H—J [wig _ J 9 51 in»! L trivial: (00” ‘3 t F it w' 3FIP'11JL“ 4 Clz’wh'gt 0‘; -'- 6“ hyu’rvrmvi; s-Lov Salaam: F P-okm wt 2, Edit-“.1 “ML hatti in “HM: “154*” JrMJM‘Mb’M‘ LLI'iECJ ‘KMMP‘rfi'gIWNE’ Efl- "-‘llfltn nnrikrr gar—l Sr‘ium L312 2007 Drummond Exam ll Your name Multiple choice (2 points each). c. GTP is hydrolyzed to GDP. leading to the rapid decay of the polymer w i d. the microtubule binds to a capping protein that allows transport Iover long distances (I N vhf , u ' I}; C 2. The following best describes how actin is polymerized P H J ,2.” the actin monomer bound to GTP adds to the plus end of the polymer ,b’. actin monomers first stack in parallel coiled coil structures, then in antiparallel structures 0. the actin monomers may be added to either end of the actin filament d. the actin grows out of an organizing body called the centrosome D 3. Which answer does NOT support the analogy between the ras protein and the Ga subunit in a G-protein? a. they both bind GDP b. they are both activated by exchange of GDP for GTP c. they are both associated with the membrane using a lipid tail that dis solves in the membrane d. they are both coupled to receptor tyrosine kinases (RTKs) A 4. This is an organelle where you would find enzymes to add carbohydrates to proteins a. The Golgi apparatus 0. The nucleus b. The lysosome d. The peroxisome l3 5. The following statement is NOT true about intermediate filaments ,a.‘ They form twisted cables using a '7 around 1' arrangement for the fibers b. Motor proteins can move along intermediate filaments to move cargo such as vesicles ,c»: Formation of the cables may be reversed by phosphorylation d. These filaments can tie cells together via structures called desmosomes D 6. The following is true of lysosomes a: They usually have a high pH (around 9) to facilitate degradation of lipids bf Specialized modifications to proteins occur here before they move to the plasma membrane E 7. The following is the most accurate depiction of how dynein walks on microtubules ,6 The to drive each step in an alternating fashion ,dc One motor protein head group binds to the intermedtatefilament, ATP binds, and the second steps forward 5 wtl L312 2007 Drummond Exam ll Your name 5; 8. The following is the largest or longest structure a. an intermediate filament monomer (length) it tnm , b. The diameter of a microtubule 1—} M... ".15 .\ .v\ c. the plasma membrane - gum d. a vesicle in the clathrin-mediated transport i a 0 mm ' D 9. This is what enforces the shape of the Golgi apparatus as a series of flattened sacs. a. membrane polymers b. actin polymers 0. intermediate filament polymers d. microtubule polymers E 10. The following is NOT true of steroid hormone receptor-mediated signaling a. The steroid hormones are hydrophobic and freely dissolve in membranes b. They bind to a transmembrane receptor and cause a conformational change cf. The steroid itself must move with its receptor into the nucleus cf. The signaling does not involve an extensive intracellular cascade fl 11. Which of the following filaments is NOT polar? a. intermediate filaments Jar} microtubules )2 actin filaments d. these are all polar filaments 12. The following best describes actin filaments that project outward in bundles to force small, finger-like extensions in the cell membrane. . pseudopodia . filopodia lamellipodia none of these really describes this phenomenon 9.0m» A 13. This best describes the structure and role of the integrin a. it is a transmembrane protein that interacts with both the actin cortex and an extracellular surface )af. It is a transmembrane protein that binds intermediate filaments and forms the interaction between two cells A 14. The following element is common to signal transduction using receptor tyrosine kinases, G-protein linked receptors, and steroid hormone receptors: a. A ligand binds to a receptor to trigger a set of downstream events b. A ligand binds to a transmembrane receptor and triggers intracellular changes 0. A nucleotide phosphate cofactor (ATP or GTP) is involved when the ligand binds the receptor ,df The Map kinase cascade is initiated. ~th K L312 200? Drummond Exam ll Your name lg 15. The following best describes motor proteins movement on structural polymers. You have to keep in mind which proteins walk on which polymer to answer the question. a. kinesin and dynein both walk to the plus end of their respective filaments b. kinesin and myosin both walk to the plus end of their respective filaments c. myosin and kinesin both walk to the minus end of their respective filaments d. myosin and dynein both walk to the minus end of their respective filaments Page totals: Page 1 __3*__ l20 Page 2 I14 1 Page 3 3i [16 Page 4 120 Total Li r100 ...
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This note was uploaded on 07/02/2011 for the course BIO-L 312 taught by Professor Drummond during the Fall '07 term at Indiana.

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KeyII-Spring 07 - / L3122007 Drummond Exam ll \g ‘l. (10...

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