Lecture 22-07web - L312/Spring 2003 Lecture 22 Drummond...

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L312/Spring 2003 Lecture 22 Drummond April 7 or today: Start Chapter 21; tissue structure and connections today (skip plant tissues) Last time: Anaphase initiation by APC (anaphase promoting complex) : triggers inhibitor degradation active protease degrades cohesins, metaphase progresses to anaphase MAPs and catastrophins in maintaining a steady state involving microtubules failure to degrade: long strings/hair failure to stabilize: short, unconnected Critically compare features of microtubules and MAPs in mitosis XKCM-1 (catastrophin) and XMAP-215 (X = xenopus, a favored frog) Finish: Steps in anaphase: anaphase A: kinetochore microtubules shorten anaphase B: interpolar microtubles lengthen and slide; centrosomes move apart The steps in mammalian cytokinesis The role of actin and myosin This time: Tissues (connective, epithelial, etc.) and the extracellular matrix What holds cells together? What gives tissues strength?
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MAPs bind microtubules and modulate stability and function XMAP215 XKCM1 Antibody depleted Antibody depleted Antibody depleted Implications: [MT]? [GTP]?
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Actin and myosin are central to cell resolution into two cells What are possible roles for the interpolar microtubules at this late stage?
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