GMOweek2 - 10/19/2009 C118 Laboratory Genetically Modified...

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10/19/2009 1 C118 Laboratory Genetically Modified Foods Week 2 Separation and Detection of DNA Full Typed Report Due one week from completion of lab Refer to lab report style guidelines The Debate Continues •P ro GMO http://www.monsanto.com/biotech- gmo/asp/videogallery.asp Anti GMO http://www.greenpeace.org/usa/
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10/19/2009 2 Overview of Experiment Purpose? 455 bp 200 bp Review from last week • Purpose of PCR was • A. Chop up DNA into small fragments • B. Make lots of copies of certain sections of DNA • C. Check for contamination with human DNA • D. Test to see if your AI’s were dedicated enough to come back and get your samples 4 hours after lab.
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10/19/2009 3 Group Work • Six samples prepared in last weeks lab: – 1) Non GMO with plant primers – 1) Non-GMO with plant primers – 2) Non-GMO with GM primers – 3) Test food with plant primers – 4) Test food with GM primers – 5) Positive GMO DNA with plant primers – 6) Positive GMO DNA with GM primers What is the purpose of each sample?
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This note was uploaded on 07/02/2011 for the course CHEM-C 118 taught by Professor Flood during the Spring '10 term at Indiana.

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GMOweek2 - 10/19/2009 C118 Laboratory Genetically Modified...

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