HW-I-2010

HW-I-2010 - uncorrupted stack (Bars.zip). Write a summary...

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1st Homework Assignment Deconvolution April 9, 2010 Please download the ImageJ software from the website at: http://rsb.info.nih.gov/ij/ Download the ImageJ plugin “DeconvolutionLab” from this website: http://bigwww.epfl.ch/algorithms/deconvolutionlab/ 1) First you will work on the dataset provided on the EPFL website, “Synthetic Hollow Bars”. http://bigwww.epfl.ch/deconvolution/?p=bars Download the dataset (use Bars-G10-P30) and the PSF (PSF-Bars.zip) and apply the following filters: 1) Inverse filter, 2) Regularized inverse filter and 3) Richardson-Lucy with 10 and 4) 30 iterations. Follow instructions on the EPFL Webpage as you do these steps. PSF selection requires to click onto PSF under Modules in Deconvolution window on left. Document with snapshots (one representative image or 3D view for each method) the improvement in image quality by comparing these four methods and with the
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Unformatted text preview: uncorrupted stack (Bars.zip). Write a summary of 4-5 sentences what you think is the best and the worst of these methods. 2) Download the datasets from the C-Elegans and the corresponding PSFs: http://bigwww.epfl.ch/deconvolution/?p=bio Note that for each channel there is a separate PSF because of differences in wavelength. Apply the following filters: 1) Inverse filter, 2) Regularized inverse filter and 3) Richardson-Lucy with 10 and 4) 30 iterations. Document with snapshots (one representative image or 3D view for each method) the improvement in image quality by comparing these four methods. Write a summary of 4-5 sentences about your experience and the performance of these methods if compared to the original image stacks. Please notice that in Image J you can combine single channels into color images....
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