4. Biotechnology

4. Biotechnology - Biotechnology What is biotechnology? Use...

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Biotechnology
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What is biotechnology? Use of living organisms to create new products 1. Transgenic biotechnology = DNA from one species and put it  into another species 2. Reproductive cloning = creating animals through cloning 3. Forensic biotechnology = use biotechnology to establish  identities 4. Personalized medicine = develop medicines that are tailored to  the individual
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1. Use restriction enzymes to cut  out your gene of interest and   cut the plasmid 2. Insert your gene into the  plasmid  3. Transfer the plasmid back into  bacterial cells = Transformation 4. Let the bacteria replicate =  transgenic  organism 5. Purify your gene 1. Transgenic Biotechnology
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Foreign DNA incorporated into their genetic material Gene target Single gene inactivated or “knocked out” “Knockout mice” used in studying genes Mutagenesis screening Male mice treated with mutagens and studied Transgenic organisms
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Transgenic Biotechnology Human growth hormone Give to children of short stature Source = cadavers                                        100 cadavers  per child to a one year supply  What other problems  could this cause?              Genentech created growth hormone through recombinant  technology
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Restriction enzymes Enzymes from bacteria Used to cut DNA molecules in specific places Enable researchers to cut DNA into manageable segments Vector molecule carrier of DNA fragment into cell Transformation: uptake of foreign DNA into cells Recombinant DNA methods
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Stick to complementary DNA
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Foreign DNA and plasmid DNA cut with same restriction enzyme Produces linear molecules with complementary single-stranded ends Recombinant DNA created by mixing so sticky ends pair DNA ligase forms covalent bonds, linking the two fragments Splicing foreign DNA into a vector
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Plasmids
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Cloning  vector  = self- replicating plasmid that  serves to transfer and  replicate genetic  material
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2. Regulatory Genetics 3. Mutations 4. Translation 5. Gene Cloning Which of the following results from inserting foreign DNA into an organism to produce a new gene combination?
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Examples of Transgenic Biotechnology 1.  Human Growth Hormone 1.  Insulin                                                              
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This note was uploaded on 06/23/2011 for the course BIO 111 taught by Professor Tatum/parker during the Spring '11 term at St. Xavier.

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4. Biotechnology - Biotechnology What is biotechnology? Use...

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