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chromatography lab - resulting in different observations...

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Jones 1 Caleb Jones Chromatography Lab 28 October 2009 The TLC Experiments The solvent most appropriate for our dye was determined to be the 2-propanol solution. The data presented below includes the retention factors for each dye on the TLC plate exposed to the 15:2 solution: Bromocresol Green Basic Fuchsia Methylene Blue Methyl Red Unknown Fluoroscein .833 .738 .357 .857 .977 .929 Class Data With the exception of one group, the entire class determined that the 2-propanol solution was the best mobile phase. While class data was not actually collected, it can be inferred that the data would have been rather similar. Any deviations among experiments could probably be attributed to natural experimental error. In addition, because different dyes may have separated in different fashions, every group may have allowed their experiments to run for different periods of time,
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Unformatted text preview: resulting in different observations. Designing a New Instrument The instrument would have to be similar to a spectrophotometer. It would have to notice a change in color. The only way the instrument could achieve this is by measuring a change in the wavelength of light being absorbed and emitted. Therefore, the experiment would begin when the wavelength corresponded with the color of the dye. The experiment would be finished when multiple unique wavelengths of light were detected. Identity of Dyes in the “Unknown” Using both the TLC plates and the column, the unknown dye appeared to be composed primar-ily of a blue dye similar to methylene blue, it contained a fair amount of a pink dye similar to methyl red. It also appeared that there was a minute amount of yellow dye mixed with the un-known. Chromatography Cartoon Jones 2...
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