Colorimetry - Jones 1 Caleb Jones Chem 114 Colorimetry...

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Jones 1 Caleb Jones Chem 114 Colorimetry Spectrophotometer The spectrophotometer is an instrument that measures the absorbance of light of a sample placed into it. The spectrophotometer contains a light source, wavelength selectors, photodetectors, and other components. The sample is placed in a clear cuvet. This allows light to pass through the cuvet without any hindrances. Great care is taken to ensure that the absorbance is not affected by any external factors. Light is shined through a sample. Some of the light is absorbed by the sample. A detector on the other end of the cuvet measures how much light the cuvet has passed through. The spectrophotometer is calibrated, or “zeroed”, at a base value using a standard solu- tion. Therefore, any absorbance measurements are relative to this base value. Absorbancies Each molecule in a homogenous solution can absorb a certain amount of light at a particular wavelength. The solutions with higher concentrations and more molecules can absorb larger
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This note was uploaded on 06/25/2011 for the course CHEMISTRY 115L taught by Professor March during the Spring '10 term at University of Alabama at Birmingham.

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Colorimetry - Jones 1 Caleb Jones Chem 114 Colorimetry...

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