3 TLC - Experiment 3 Thin Layer Chromatography A...

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Experiment 3 Thin Layer Chromatography A. Introduction In this experiment, thin layer chromatography was used to identify an unknown sample by comparing different R f values for different substances. Thin layer chromatography separates components of mixtures based on the affinities for a mobile phase versus a stationary one. The stationary phase is usually a very thin layer of polar adsorbent coated on a glass or plastic plate; the mobile phase is a solvent that travels up the chromatography plate via capillary action. The more strongly adsorbed the component is to the stationary phase, the slower it moves through the system. The rate at which the compound moves depends on its polarity. The R f value is calculated by using the following formula: = Rf distance traveled by t h e compounddistance traveled by t h e solvent front Compounds separated in this experiment: Acetylsalicylic acid (aspirin) Acetaminophen (Tylenol®) Caffeine
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Phenacetin 2-(4-Isobutylphenyl)propanoic acid (Ibuprofen) Sodium 2-(6-methoxy-2- naphthyl)propanoate (Naproxen sodium) B. Experimental Reagent/Solven t Molecular Weight (g/mol) acetylsalicylic acid 180.157 caffeine 194.19 2-(4- isobutylphenyl) propanoic acid 206.28 acetaminophen 151.17 phenacetin 179.216 Four TLC plates were obtained and a line was made with pencil one cm from the edge of the plate. Two developing jars were made by placing a piece of filter paper in the jars. A small
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3 TLC - Experiment 3 Thin Layer Chromatography A...

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