Chapter 7 - 1. Gene Map a. Indicates when genes occur on a...

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1. Gene Map a. Indicates when genes occur on a chromosome, their order on the chromosome and their distance apart b. Two genes located on the same chromosome are syntenic c. Chromosomal locations of a gene i. Longarm/shortarm ii. Indentified banding patterns 1. Have distinct regions and bands and sub-bands
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2. 4 ways of gene maping a. Somatic Cell Hybridization- used to find which
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chromosome a gene is on b. Fluorescent In Situ Hybridization- used to find exact location of a gene c. Deletion Maping- used to find a region on a chromosome where a gene is d. Genetic Recombination- most accurate method to map genes 3. Somatic Cell Hybridizatoin a. Cells in culture will occasionally fuse i. The cells don’t have to be from the same species b. Cell Fusion - Initially a fused cell has 2n chromosomes from both cells (Heterokaryote) c. The cell tends to lose chromosomes during subsequent mitotic divisions before eventually stabilizing (which chromosomes are lost are random) d. Result: a series of clones with different subsets of the original double set of chromosomes e. Ways to increase the rate of Fusion i. Sendai Virus 1. Inactivated virus that enhances the rate of cell fusion 100 to 1000 times 2. Polyethylene glycol also helps cell fusion f. Ways to ensure that only hybrid cells survive i. Human fibroblasts are hprt- and need additional media to survive ii. Mouse tumor cells are tk- and need additional media iii. Hybrid cells are tk+ and HPRT+ and can survive in normal HAT medium g. Human-mouse hybrid cells i. Tend to loose mostly human chromosomes
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ii. A human cell that lost most of its chromosomes would die iii. A hybrid cell is viable as long as all the important genes are presnt (either on human or mouse chromosomes 4. Fluorescent In Situ Hybridization (Fish) a. Clone or sequence the part of a gene
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This note was uploaded on 06/28/2011 for the course BY 210 taught by Professor Powell during the Summer '08 term at University of Alabama at Birmingham.

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Chapter 7 - 1. Gene Map a. Indicates when genes occur on a...

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