Chapter 12 - Chapter 12 DNA Replication and Recombination 1 a Possible mechanisms of replication b Meselson-stahl experiment 2 Theta Replication a

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Chapter 12: DNA Replication and Recombination 1. Possible mechanisms of replication a. b. Meselson-stahl experiment 2. Theta Replication a. Replication in e.coli
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There is a single origin of replication c. The unwinding of the double helix generates a loop, termed a replication bubble. d. Replication proceeds around the circle in both directions 3. Rolling circle replication a. Takes place in some viruses and in the F factor of E.coli 4. Linear Eukaryotic Replication a. DNA polymerase i. Enzymes that synthesize DNA ii. Requires the following: 1. All four nucleic acids to be present 2. A strand of template DNA 3. A strand of DNA with a 3’ end open 4. Can only add nucleotides to the 3’ end of the growing strand 5. DNA synthesis always happens 5’ to 3’ iii. 2 terminal phosphates from the nucleotides are cleaved iv. The remaining phosphate is linked to the 3’ end of the growing chain to form a phosphodiester bond. v.
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This note was uploaded on 06/28/2011 for the course BY 210 taught by Professor Powell during the Summer '08 term at University of Alabama at Birmingham.

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Chapter 12 - Chapter 12 DNA Replication and Recombination 1 a Possible mechanisms of replication b Meselson-stahl experiment 2 Theta Replication a

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