chapter 14 - Chapter 14: RNA molecules and RNA processing...

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Chapter 14: RNA molecules and RNA processing 1. In most prokaryotes, the product of transcription is the mRNA. Translation begins even before synthesis of the mRNA is completed 2. Producing a protein in a eukaryote requires processesing and transportaotion 3. RNA processing in eukaryotes a. A “cap”(methylated guanosine) is added to the 5’ end of the RNA transcript. b. A poly-A tail (150-200 nucleotides) is added to the 3’ end c. Splicing occurs 4. 5’ cap
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5. Poly- A tail 6. Split Genes a. Most eukaryotic genes contain segments that are spliced out of the RNA before the final, shorter, mRNA is translated. b. Introns i. “intervening sequences” which will be removed ii. Does not code for anything c. Exons i. Will remain as part of the mRNA and be expressed as a protein d. When introns are removed, it is critical that splicing occur at exactly the right place. If the cut is off by a single nucleotide, the resulting mRNA will have the wrong nucleotide sequence e. Introns contain 3 consensus sequences critical to splicing
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This note was uploaded on 06/28/2011 for the course BY 210 taught by Professor Powell during the Summer '08 term at University of Alabama at Birmingham.

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chapter 14 - Chapter 14: RNA molecules and RNA processing...

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