Colorimetry - Colorimetry By: David Mulkey 1. The...

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Colorimetry By: David Mulkey 1. The spectrophotometer is a device that is used to measure absorbance. The spectrophotometer is an instrument in the lab that sends light at a certain wavelength through a solution to measure the absorbance. Then it sends the measurement to the computer, so the result can be viewed. It works by first setting the wavelength needed to measure the absorbance. In this case, it was 560 nanometers. Then the instrument must be zeroed by using a reference solution which is used to subtract out the absorbance due to the other species in solution. Then the sample of solution in a cuvet will be placed in the cell compartment of the spectrophotometer. At this point, start the spectrophotometer which sends the light through the sample solution and observe the absorbance value. 2. Our stock solution had a .02 molarity which represents the concentration of the solution, so as the dilutions were performed the solution became less and less concentrated. The absorbance measurement for the stock solution was .8608, but
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This note was uploaded on 06/29/2011 for the course CHEMISTRY 115L taught by Professor March during the Spring '10 term at University of Alabama at Birmingham.

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Colorimetry - Colorimetry By: David Mulkey 1. The...

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