Chromatography - Chromatography By: David Mulkey 1. In the...

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Chromatography By: David Mulkey 1. In the experiment, five known dyes and an unknown dye were placed on a thin layer chromatography plate. Each dot was evenly placed one centimeter from each other on a pencil line that was a centimeter from the bottom edge of the thin layer chromatography plate. Then the plate was placed in a solvent that ran up the plate moving the dyes allowing us to acquire our results. Dye Number of spots Color of spots R f Value Solvent (1:1:1) New Methylene Blue 1 Blue .35 Water:propanol:acetone Methyl Red 2 Orange and yellow Orange=.83 Yellow=.79 Water:propanol:acetone Fluorescein 1 Purple .94 Water:propanol:acetone Basic Fuchsin 1 Yellow .94 Water:propanol:acetone Bromocresol Green 1 Green .97 Water:propanol:acetone Unknown (421) 1 Blue .35 Water:propanol:acetone R f value means the retention factor value. 2. The retention factor values as well as the color of the spots of each dye needs to be recorded according to the solvent that was used. The groups used the same
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This note was uploaded on 06/29/2011 for the course CHEMISTRY 115L taught by Professor March during the Spring '10 term at University of Alabama at Birmingham.

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Chromatography - Chromatography By: David Mulkey 1. In the...

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