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lec23 - Lecture 23 Bacterial genetics I Prokaryotes an...

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Lecture 23 – Bacterial genetics I. Prokaryotes – an overview A. genetic material in region lacking clear boundaries B. DNA is circular molecule C. three modes of DNA transfer  D. advantages of bacteria 1. haploid  2. generation time 3. easy to grow 4. individual members of population  E. growth 1. on agar 2. can grow in liquid broth F. types of mutants 1. antibiotic resistant 2. nutritional mutants a. prototroph –  can grow in simple medium of few salts & carbon source b. auxotroph – cells that can’t grow on minimal medium 3. carbon-source mutants II. Conjugation A. form of DNA transferred 1
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Picture 68 1. episome – genetic element that can exist free in cell or  integrated into chromosome - plasmid 2. chromosome B. mating involves transfer of F - conjugation schematic C. Hfr - high frequency of recombination D. Hfr mating: 2
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Picture 71 E. a few points
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