lec29 - Picture 109 Lectures 29 and 30 applications of...

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Picture 109 Picture 1 0 Lectures 29 and 30 – applications of  recombinant DNA technology I. Manipulate gene of interest A. site-directed mutagenesis B. in vitro mutagenesis by PCR why? C. reporter transgenes - fuse regulatory sequences to reporter - use  lacZ  (encodes  β -galactosidase),  gfp  (jelly fish green fluorescent protein) or  luciferase (fire fly enzyme) 1
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Picture 1 1 Picture 1 2 Picture 1 9 - eg:  ceh-23::gfp   - why? II. Transgenic organisms  - introduce gene from one species into genome of second species A. transgenic bacteria – generally  introduce gene on plasmid - why? 2
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Picture 121 Picture 12 Picture 123 B. transgenic yeast 1. how? 2. uses of yeast transformation C. transgenic plants 1. can be transformed by particle gun or by  Agrobacterium  Ti plasmid 2. Ti plasmid:  3
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Picture 124 3. engineering plants 4. types of engineered plants a. luciferase (from firefly) b. b-galactosidase (from 
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This note was uploaded on 07/01/2011 for the course L 311 taught by Professor Forrester during the Fall '10 term at Indiana State University .

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lec29 - Picture 109 Lectures 29 and 30 applications of...

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