lec29 - Picture 109 Lectures 29 and 30 applications of...

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Picture 109 Picture 110 Lectures 29 and 30 – applications of  recombinant DNA technology I. Manipulate gene of interest A. site-directed mutagenesis B. in vitro mutagenesis by PCR why? C. reporter transgenes - fuse regulatory sequences to reporter - use  lacZ  (encodes  β -galactosidase),  gfp  (jelly fish green fluorescent protein) or  luciferase (fire fly enzyme) 1
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Picture 111 Picture 112 Picture 119 - eg:  ceh-23::gfp   - why? II. Transgenic organisms  - introduce gene from one species into genome of second species A. transgenic bacteria – generally  introduce gene on plasmid - why? 2
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Picture 121 Picture 122 Picture 123 B. transgenic yeast 1. how? 2. uses of yeast transformation C. transgenic plants 1. can be transformed by particle gun or by  Agrobacterium  Ti plasmid 2. Ti plasmid:  3
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Picture 124 3. engineering plants 4. types of engineered plants a. luciferase (from firefly) b. b-galactosidase (from  E. coli
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