lec31 - Lectures 31 and 32 Genome analysis I. Genome...

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Picture 134 Lectures 31 and 32 – Genome analysis I. Genome analysis A. two general areas 1. structural 2. functional B. genome projects – a status report 1. 1 st  sequenced: several viral genomes 2. mitochondria and chloroplasts 3. many bacteria 4. many complete nuclear genomes have been sequenced C. why perform whole genome analyses? 1. genomic DNA is blueprint for making organism 2. comparison – shows important sequences 3. human genomics – clone genes associated with genetic disorders  4. many projects generate ordered clones that cover genome 1
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Picture 121 Picture 12 D. general strategy 1. position genes and molecular markers along chromosome map 2. position individual cloned DNA fragments relative to one another 3. sequence DNA 4. assemble sequence II. Clone genome into workable sized pieces A. goal is ordered set of overlapping clones that together cover the entire genome 1. first clone large pieces into YACs (or BACs, PACs) 2. order the clones into contigs a. by FISH (fluorescence in situ  hybridization) b. by sequence tagged sites (STSs) - unique sites on chromosome, often specific PCR products - if from cDNAs, they’re called expressed sequence tags (ESTs) 2
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This note was uploaded on 07/01/2011 for the course L 311 taught by Professor Forrester during the Fall '10 term at Indiana State University .

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lec31 - Lectures 31 and 32 Genome analysis I. Genome...

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