probset09 - Problem Set 9 Fall 2010 Name 8 points total 1 A...

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Problem Set 9, Fall, 2010 Name: 8 points total 1. A Hfr strain possessing the markers his + met + tyr + str s was mated with a F strain possessing the markers his met tyr str r . The time of entry for each marker is shown below. Donor Marker Time (min) his + 21 met + 12 tyr + 46 a. Based on the results draw a map of the Hfr chromosome. Indicate the position of the F element, and the first marker to be transferred. b. On what type of plates were cells grown to determine the above information? 2. In three different interrupted mating experiments, you obtain the following data for the time of entry of the following genes: arg , met , leu , ser , gal , val , and phe . All of these experiments were conducted using Hfr strains (Hfr 1, Hfr 2, and Hfr3) derived from the same original strain of E. coli . Experiment 1 (Hfr 1) Experiment 2 (Hfr 2) Experiment 3 (Hfr 3) Met 10 min Val 25 min Leu 30 min Arg 40 min Ser 55 min Gal 60 min Gal 5 min Ser 10 min Leu 35 min Val 40 min Met 55 min Phe 85 min Leu 5 min Val 10 min Met 25 min Phe 55 min Gal 75 min Ser 80 min a. Determine the order of these genes on the bacterial chromosome and draw a single map showing the position
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