probset12 - 4.0kb 3.5kb Problem Set 12, Fall 2010 Name: 8...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
4.0kb 3.5kb 2.5kb 2.0kb 1.0kb 0.2kb Problem Set 12, Fall 2010 Name: 8 points total 1. A rare autosomal recessive disorder causes pink hair. A non-carrier male and an affected female marry. Consider the following pedigree and RFLP analysis prepared using Hin dIII, Southern blot, and a probe with a human sequence known to hybridize to sequences closely linked to the gene responsible for this disorder. a. Diagram each of the different haplotypes (group of markers that tend to be transmitted together because of linkage) of I-1 and I-2 showing the restriction map and fragment lengths. Show all Hin dIII restriction sites on each map. b. On the restriction map, indicate the area to which the radioactive probe must hybridize in order to detect the fragments seen by Southern analysis. c. To which haplotypes (essentially pattern of each chromosome) does the mutant allele of the gene seem to be linked in this family. d. Why does individual III-1 only show one band in the RFLP analysis? e. Which individuals are probably carriers? 1
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Problem Set 12, Fall 2010 Name: 8 points total 2. Your friend just got a new job in a research lab in Brazil studying tropical butterflies. She has recently isolated a new mutant butterfly that produces fluorescent blue wings. She calls the
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 5

probset12 - 4.0kb 3.5kb Problem Set 12, Fall 2010 Name: 8...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online