Ch28 - Chapler 28 Nucleic Acids S olutions 766 C hapter 28 Nucleic Acids 767 Solutions Problem 28 4 Here is a pQrtiQn Qf the nucleQtide sequence in

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766 Solutions Chapler 28: Nucleic Acids CHAPTER 28 Solutions to the Problel1)S PrQblem 28.1 Draw a structural fQrmula fQr each nucleQtide. (a) 2'-DeQxythymidine 5'-mQnQphQsphate 0 .. =r Phosphoric CH 3 HN ester bond I I 0/ O~N' II 5'~ ~·N·Glycosidic -cr-:-P-o-CH 2 0 - bond. 6 4' H H I' - H H 3' 2' HO H (b) 2'-DeQxythymidine 3'-mQnQphQsphate- o HN~CH3 oA ) N HOCHW' , - ~.N·Glycosidic 2 0 bond 4' H H ,I" • ,H 3' ,2' H Phosphoric ' H ester bond ------ I -o-P=O I o Problem 28,2 Draw a structural fQrmula fQr the sectiQn Qf DNA that contains the base sequooce CTG and is phQsphQry!attid Qn the 3' end Qnly. 3'End PrQblem 28,3 Write the cQmplementary DNA base sequence fQr ~'-CCGTACGA-3'. The complementary sequence would be ~'·GGCATGCT·5' Chapter 28: Nucleic Acids Solutions 767 Problem 28 4 Here is a pQrtiQn Qf the nucleQtide sequence in phenylalanine tRNA. 3'-ACCACCUGCUCAGGCCUU-5' Write the nucleQtide sequence Qf its DNA cQmplement. Remember that ~he base uracil (U) in RNA is complementary to adimine (A) in DNA. The complement DNA sequence of the above RNA sequence would be: 5'·TGGTGGACGAGTCCGGAA·3'. Problem 28 5 The fQllQwing sectiQn Qf DNA cQdes for QxytQcin, a pQlypeptide hQrmQne. 3'-ACG-ATA-TAA-GTI-TIA-ACG-GGA-GAA-CCA-ACT-5' (a) Write the base sequence Qf th~ mRNA synthesized frQm this sectiQn Qf DNA. , The base sequence of the mRNA synthesized from'this section of DNA would be: 5'·UGC·UAU·AUU·CAA·AAU·UGC·CCU·CUU·GGU·UGA·3' (b) Given the sequence Qf bases in part (a), write the primary structure Qf QxytQcin. The primary sequence of oxytocin would be: Amino terminus· Cys·Tyr·I1e·Gln·Asn·Cys·Pro·Leu·Gly ·Carboxyl terminus Note how the last codon, UGA, does not code for an amino acid, but rather is the stop signal. Problem 28,6 The fQllQwing is anQther sectiQn Qf the bovine rhQdopsin gene. Which Qf the endQnucleases given in Example 28.6 will catalyze cleavage'Qf this sectiQn. FnuDll lIpaII 51-ACGTCGGGrCGTCGTCCTC~GGTCAGTC~TCTTcr -3' The FnuDII and Hpall cleavage sites are,shown on the sequence above. PROBLEMS , Nucleosides and Nucleotides ' Problem 28.7 A piQneer Qf designing and synthesizing antimetabQJites that CQuld destrQy cancer cells was GeQrge' Hitchings at BurrQughs WellcQme CQ. In 1942 he initiated a prQgram tQ discQver DNA.antimetabQlites, and in 1948 he and Gertrude EliQn synthesized 6-mercaptQpurine, a s\lccessful dt:Ug fQr treating acute leukemia. AnQther DNA antimetabQlite synthesized by Hutchings,andEliQn was 6-thiQguanine. Hitchings and EliQn alQng with Sir James W. Black WQn the 1988 NQbel Prize fQr PhysiQIQgy Qr Medicine fQr their discQveries Qf "impQrtant principles of drug treatment". In each drug, the Qxygen at carbQn 6 Qf the parent mQlecule is replaced by divalent sulfur. Draw structural fQrmulas fQr the enethiQI (the ,sulfur equivalent Qf an enQI) fQrms Qf 6-mercaptQpurine and 6-thiQguanine. ' .
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This note was uploaded on 07/09/2011 for the course CHE 301 taught by Professor Diver during the Spring '08 term at SUNY Buffalo.

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Ch28 - Chapler 28 Nucleic Acids S olutions 766 C hapter 28 Nucleic Acids 767 Solutions Problem 28 4 Here is a pQrtiQn Qf the nucleQtide sequence in

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