microbio lab report-1

microbio lab report-1 - DETATILED ANALYSIS AND...

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DETATILED ANALYSIS AND IDENTIFICATION OF THE GRAM NEGATIVE BACILLI, PROTEUS VULGARIS Abstract: Proteus Vulgaris , a part of the enterobacteriaceae family is predicted to be the unknown bacteria # D by performing a series of tests. The types of tests that were used to test the unknown bacteria # D consisted everything from lactose, glucose, phenylalanine, methyl red, voges-prokauer, indol production, caltalyase, hydrogen sulfide, urease, motility, acid production, nitrate reduction, EMB, and SAL-SHIG agar plates. These tests are proven positive or negative either by how many colonies the agar plates contain, or by the color change detected in the nutrient broths, or slants. This color change ranged from simply looking at the incubated test tubes to adding additional reagents to the slants/broths to confirm if the test was positive or negative. Kirby Bauer experiment was also an important part of the unknown process as it identified what antibiotic the unknown bacteria was resistant to, and which antibiotic the bacteria was sensitive to. The gram stain was also used to help identify the bacteria as gram positive or gram negative. Overall, the main aim of this experiment was to identify the unknown bacteria given to us and explain the process is carried out by the elimination technique. Introduction: Keeping the main objective of the experiment in mind, the unknown bacteria D was closely predicted with the bacteria Proteus Vulgaris . The Proteus species are part of the Enterobacteriaceae family of gram- negative bacteria. Proteus organisms are often implicated as causes of infections in humans, along with Escherichia, Klebsiella, Enterobacter, and Serratia species. The Proteus species are most commonly found in the human intestinal tract as part of normal human intestinal flora, along with Escherichia coli and Klebsiella species, of which E coli is the predominant resident. Proteus species possess an extracytoplasmic outer membrane, which is also a feature shared with other gram-negative bacteria. In addition, the outer membrane contains a lipid bilayer, lipoproteins, polysaccharides, and lipopolysaccharides. Infection depends on the interaction between the infecting organism and the host defense mechanisms. These species also ferment sugars in anaerobic conditions but can use a wide range of organic molecules in aerobic conditions. Some Proteus
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species are mainly considered soil inhabitants and is particularly common in decomposing organic matter. However, other Proteus species and the related genus Providencia can quite frequently cause urinary tract infections. In the given experiment, the unknown bacteria # D was identified using many steps of tests. First the simple steps were used such as preparing a gram stain or creating the Kirby Bauer experiment. Then the real analysis came down to identifying the color changes in the tubes and identifying with the agar plates. In total, this unknown identification took approximately 2 weeks. The first week dealt with performing the initial
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This note was uploaded on 04/04/2008 for the course MICRO 118 taught by Professor St.john during the Spring '07 term at Rutgers.

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microbio lab report-1 - DETATILED ANALYSIS AND...

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