Microbio Unknown

Microbio Unknown - Sharon Santos BIO 409 April 27, 2011...

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Sharon Santos April 27, 2011 BIO 409 Identification of Two Unknown Bacterial Specimen from Unknown Two Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. . Isolation and identification techniques are important aspects of microbiology. Applications include clinical diagnoses, evaluation of water quality, and discovery of new microbial species. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function. Methods After streaking out the bacteria and isolating two unique organisms, the organisms were gram stained and identified to be a gram positive cocci and a gram negative rod. In order to identify the gram positive cocci the following tests were performed: catalase, coagulase, and mannitol salt agar. The identification of the gram negative rod required a few more tests and the following tests were performed to identify the unknown: motility, oxidase, gelatin, phenol red, decarboxylase, citrate and hektoen enteric agar. After performing these tests, the two unknowns were able to be identified. Results Table 1: Tests Performed on Gram Positive Cocci Test Results Interpretation Gram Stain Purple Gram Positive Cocci Catalase Test Bubbles Catalase is present. Organism produces the
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Microbio Unknown - Sharon Santos BIO 409 April 27, 2011...

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