Microbio Unknown - Sharon Santos BIO 409 Identification of...

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Sharon SantosApril 27, 2011BIO 409Identification of Two Unknown Bacterial Specimen from Unknown TwoIntroductionThe purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. . Isolation and identification techniques are important aspects of microbiology. Applications include clinical diagnoses, evaluation of water quality, and discovery of new microbial species. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function. MethodsAfter streaking out the bacteria and isolating two unique organisms, the organisms were gram stained and identified to be a gram positive cocci and a gram negative rod. In order to identify the gram positive cocci the following tests were performed: catalase, coagulase, and mannitol salt agar. The identification of the gram negative rod required a few more tests and the following tests were performed to identify the unknown: motility, oxidase, gelatin, phenol red, decarboxylase, citrate and hektoen enteric agar. After performing these tests, the two unknowns were able to be identified.ResultsTable 1: Tests Performed on Gram Positive CocciTestResultsInterpretationGram StainPurpleGram Positive CocciCatalase TestBubblesCatalase is present.

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